| 1. Identity statement | ||||
| Reference Type | Journal Article | |||
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S | |||
| Site | mtc-m21d.sid.inpe.br (namespace prefix: upn:44QHRCS) | |||
| Identifier | 8JMKD3MGP3W34T/46LDT9B | |||
| Repository | sid.inpe.br/mtc-m21d/2022/04.06.19.18 (restricted access) | |||
| Last Update | 2022:04.06.19.18.56 (UTC) simone | |||
| Metadata Repository | sid.inpe.br/mtc-m21d/2022/04.06.19.18.56 | |||
| Metadata Last Update | 2023:01.03.16.46.04 (UTC) administrator | |||
| DOI | 10.1016/j.carpta.2022.100198 | |||
| ISSN | 2666-8939 | |||
| Citation Key | MartinsMoMoArMaPaEs:2022:DeStKe | |||
| Title | Determination and standardization of the kefiran extraction protocol for possible pharmacological applications  | |||
| Year | 2022 | |||
| Month | June | |||
| Access Date | 2026, Nov. 12 | |||
| Type of Work | journal article | |||
| Secondary Type | PRE PI | |||
| Number of Files | 1 | |||
| Size | 18611 KiB | |||
| 2. Context | ||||
| Author | 1 Martins, Eduardo Ferreira 2 Moura, Nayara Koba de 3 Moura, Thales Koba de 4 Araújo, Ticiana Vasques 5 Machado, João Paulo Barros 6 Passador, Fábio Roberto 7 Esposito, Elisa | |||
| Resume Identifier | 1 2 3 4 5 8JMKD3MGP5W/3C9JHER | |||
| Group | 1 2 3 4 5 COPDT-CGIP-INPE-MCTI-GOV-BR | |||
| Affiliation | 1 Universidade Federal de São Paulo (UNIFESP) 2 Universidade Federal de São Paulo (UNIFESP) 3 Universidade Federal de São Paulo (UNIFESP) 4 Universidade Federal de São Paulo (UNIFESP) 5 Instituto Nacional de Pesquisas Espaciais (INPE) 6 Universidade Federal de São Paulo (UNIFESP) 7 Universidade Federal de São Paulo (UNIFESP) | |||
| Author e-Mail Address | 1 eduardofm1996@outlook.com.br 2 3 4 5 machadopaulo@gmail.com | |||
| Journal | Carbohydrate Polymer Technologies and Applications | |||
| Volume | 3 | |||
| Pages | e100198 | |||
| Host Collection | urlib.net/www/2021/06.04.03.40 upn:44QHRCS | |||
| History (UTC) | 2022-04-06 19:19:39 :: simone -> administrator :: 2022 2023-01-03 16:46:04 :: administrator -> simone :: 2022 | |||
| 3. Content and structure | ||||
| Is the master or a copy? | is the master | |||
| Content Stage | completed | |||
| Transferable | 1 | |||
| Content Type | External Contribution | |||
| Version Type | publisher | |||
| Keywords | Biopolymer Exopolysaccharide Fermentation Kefir Kefiran | |||
| Abstract | The use of biopolymers in medicine and pharmacology is of great scientific, technological, and commercial interest. A possibility of biopolymer for use in pharmacological applications is the kefiran, an exopolysaccharide (EPS) produced by bacteria that form kefir grains during the milk fermentation process in which the grains are growing. The EPS extraction method can modify its concentration and structure. To establish the most appropriate kefiran extraction protocol and standardize this method, three different extraction processes were evaluated, which differ in the boiling times and in the presence (or not) of agitation. Kefiran obtained by different methods were characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), electron scanning field emission microscopy (FESEM), and determination of antimicrobial potential. The samples showed differences in yield, chemical composition, degree of purity, thermal properties, and antimicrobial capacity. The results analyzed indicated that extraction protocol 1 (EP 1) with a longer heating time and constant agitation was able to provide the best thermal resistance, with a high degree of purity (93%), and yield (22.18 mg of kefiran obtained for each gram of kefir grains used). In addition, this method provides good biological activity as a potential antimicrobial agent and can be a potential biopolymer for pharmacological application. | |||
| Area | FISMAT | |||
| doc Directory Content | access | |||
| source Directory Content | there are no files | |||
| agreement Directory Content |
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| 4. Conditions of access and use | ||||
| Language | en | |||
| Target File | martins_2022_determination.pdf | |||
| User Group | simone | |||
| Reader Group | administrator simone | |||
| Visibility | shown | |||
| Read Permission | deny from all and allow from 150.163 | |||
| Update Permission | not transferred | |||
| 5. Allied materials | ||||
| Next Higher Units | 8JMKD3MGPCW/46KUES5 | |||
| Citing Item List | sid.inpe.br/bibdigital/2022/04.03.23.11 - 81 sid.inpe.br/mtc-m21/2012/07.13.14.50.56 - 12 | |||
| Dissemination | PORTALCAPES; SCOPUS. | |||
| 6. Notes | ||||
| Empty Fields | alternatejournal archivingpolicy archivist callnumber copyholder copyright creatorhistory descriptionlevel e-mailaddress format isbn label lineage mark mirrorrepository nextedition notes number orcid parameterlist parentrepositories previousedition previouslowerunit progress project rightsholder schedulinginformation secondarydate secondarykey secondarymark session shorttitle sponsor subject tertiarymark tertiarytype url | |||
| 7. Description control | ||||
| e-Mail (login) | simone | |||
| update | ||||